Bioplastic from food waste liquid fraction With a focus on food waste utilisation
نویسندگان
چکیده
.................................................................................................................................................. 2 Introduction ............................................................................................................................................ 4 Materials and methods ........................................................................................................................... 6 Chemicals and reagents ...................................................................................................................... 6 Strain ................................................................................................................................................... 6 Media .................................................................................................................................................. 6 Cultivations ......................................................................................................................................... 7 Analyses .............................................................................................................................................. 9 Results ................................................................................................................................................... 10 Food-waste medium preparation ..................................................................................................... 10 Cultivations ....................................................................................................................................... 13 Reference cultures on minimal salt medium ................................................................................ 13 Food-waste medium cultivations .................................................................................................. 14 Production of PHB on food-waste medium .................................................................................. 20 Discussion.............................................................................................................................................. 22 Goal and summary ............................................................................................................................ 22 Food waste characteristics; sterility, analysis and fat. ..................................................................... 22 Lab versus large scale........................................................................................................................ 23 Food waste as nutrient source .......................................................................................................... 23 Physiology of E. coli on food-waste medium; growth, diauxic growth and cell lysis. ...................... 23 PHB production ................................................................................................................................. 24 Outlook ............................................................................................................................................. 25 Conclusion ............................................................................................................................................. 25 Acknowledgement ................................................................................................................................ 26 References ............................................................................................................................................ 27 Master thesis 30 credits Bioplastic from food waste liquid fraction Emil Sundäng Peters
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